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| Genomic
DNA Mini-prep System |
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Genomic DNA Mini-prep System provides an easy and rapid way to purify
high quality genomic DNA from a variety of samples including blood,
animal tissues, plant, cultured cells, and bacterial cells. The purified
DNA is suitable for applications in the analysis of PCR, southern
blot, apoptosis analysis, RAPD, AFLP or RFLP, but not suitable for
applications in DNA library construction.
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Features |
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After
lysis of cells, one mini-prep can be completed in 15กซ30 minutes, and
96-well mini-prep in one hour
No phenol and chloroform extraction. No ethanol or isopropanol precipitation
Free from contamination with proteins, RNA or polysaccharides
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Principle and Procedure |
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Buffer G-A, containing strong protein denaturant, is used to lyse
cells and to release genomic DNA, meanwhile, inactivate DNase in
cells immediately. RNA is removed by digestion with RNase A1. Buffer
G-B enables solution to form two-phase system by which proteins,
carbohydrates and pigments are removed. After addition of Buffer
G-C genomic DNA in the lower phase selectively binds to silica membrane.
The impurities and salts on the membrane are removed by washing
with Buffer W1 and Buffer W2. The purified genomic DNA is then eluted
in H2O or 2.5 mM Tris-HCl, pH8.5 buffer, and can be used immediately.
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Applications
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purified DNA is suitable for analysis of PCR amplification, Southern
blotting, RAPD, AFLP, RFLP and etc. |
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